Comparative Study of Acetylation Reactions to Mentol Compounds Using Lipase fromCandida Antarctica Recombined Aspergillus oryzae with Rhizomucor miehei
Keywords:Acetylation mentol, Lipase Enzyme, Rhizomucor miehei, Candida antarctica
AbstractResearch about comparison an enzyme lipase of rhizomucormiehei and candida antarctica recombined aspergillusoryzae to catalyzes reaction asetilasi compound menthol by the difference of activity of the enzyme.Reaction done on condition temperature 50oc use up acetyl acetic anhidrid in a solvent n-heksan in a variety time.The results that is reaction asetilasi menthol use an enzyme lipase of candida antarctica recombined aspergillusoryzae and rhizomucormiehei by a unit activity different do not have a significant difference.The difference of the results the final product that is mentil acetate of these enzymes changed as from time to time produce the difference of the results at toÂ 24 hours .The use of lipase of candida antarctica recombined aspergillusoryzae have a selectivity higher ( 96,93 % ) in forming mentil acetic than lipase of rhizomucormiehei with have the ability convert of 77,42 %.
Pawiroharsono, S., 2008, Application of Enzymes for Eco-Friendly Tanning, Journal of Environmental Engineering, Vol. 9, No. 1, 51-58.
Utomo, M. P., 2010, Green Chemistry with Chemical Catalysis, National Seminar on Research, Yogyakarta, May 15, 2010.
Zhang, D., Yuwen, L. and Peng, L., 2013, Parameters Affecting The Performance of Immobilized Enzyme, Journal of Chemistry, Vol. 2013, 2-7.
Madamwar, D., 2008, Reusability of Surfactant-coated Lipase Immobilized in Gelatin Microemulsion-based Organogels for Ethyl Isovalerate Synthesis, Journal of Microbiology Biotechnology, Vol. 18, No. 4, 735-741.
Usawatun, N. C., Retnowati, R., Suratmo, 2014, Esterification of l-Mentol and Acetate Anhydride with Variation of Reactant Mole Ratio, Chemical Student Journal, Vol. 1, No. 2, 276-282.
Roji, M., JLL, Poh and Ng.T., Soo, 2001, Enantioselective Synthesis of (-) - Menthyl Acetate In a Batch Stirred Tank Reactor: Effect of Feed Batch Feeding Strategy, Symposium of Malaysian Chemical Engineers SOMCHE 2001, (E2-3).
Sri, V. H. A., 2016, Study of Acylated Asylated Reaction of Lipase Rhizomucor miehei Compound on Isopulegol Compound, Thesis, Chemistry Department, FMIPA, Universitas Brawijaya, Malang.
Kumar, A. S., Chandra, S., Dutt, P., Lal, B. K., Tava, A., and Avato, P., 1999, Essential Oil Composition of Mentha x piperita L. from Different Environments of North India, Flavor and Feagrance Journal, Vol. 14, 5-8.
Padalia, RC, Verma, RS, Chauhan, A., Sundaresan, V., and Chanotiya, CS, 2013, Essential Oil Composition of Sixteen Elite Cultivars of Menthas from Western Himalayan Region India, Journal of Science and Technology, Vol. . 7, No. 01, 83-93.
Alankar, S., 2009, A Review on Peppermint Oil, Asian Journal of Pharmaceutical and Clinical Research, Vol. 2, No. 2, 27-33.
Kadarohman, A., Essential Oils As Teaching Materials in Chemical Learning Process, 1-17.
Putri, A. R., 2017, Study of Hydrolysis Reactions L-Menthil Acetate Catalytic Enzyme Lipase Rhizomucor miehei, Thesis, Chemistry Department, FMIPA, Universitas Brawijaya, Malang.
Souza, MAA, Lemos, MJ, Brito, DMC, Fernandes, MS, Castro, RN and Souza, SR, 2014, Production and Quality of Menthol Mint Essential Oil and Antifungal and Antigerminative Activity, American Journal of Plant Science, Vol. . 5, 3311-3318.
Anonymous, L-Menthyl Acetate, www.chemicalland21.com.
Garrett, R. and Grisham, C.M., 2013, Biochemistry, 5th edition, Brooks / Cole Cengage Learning, Belmont, CA.
Wulan, P. P. D. K., Rejoso, M. T., Hermansyah, H., 2007, Reaction Hydrolysis of Olive Oil Using Lipase, Thesis, Department of Chemical Engineering, Faculty of Engineering, University of Indonesia, Depok.
Sharma S. and Kanwar, S. S., 2014, Organic Solvent Tolerant Lipases and Applications, Scientific World Journal, Vol. 2014, 1-14.
Yuanita, E., Ryantin, E., Gunawan, Kurniawati, L., Raudhatul S. K., Kinetic Studies Reaction Esterification Enzymatic Miristat Acid with Oleil Alcohol, Molecule, Vol. 8, No. 1, 9-19.
Busto, E. and Gotor, V., 2006, Candida interctica Lipase B: An Ideal Biocatalyst for the Preparation of Nitrogenated Organic Compounds, 797-812.
Ariefin, M., 2016, Reaction Studies of Cyclization Acylated Acylation of Lipase Enzyme Rhizomucor miehei to the Mixture of Sitronelal and Isopulegol Compounds, Thesis, Chemistry, FMIPA, Universitas Brawijaya, Malang.
Rodrigues R. C. and Fernandez-lafuente, R., 2010, Enzymatic Lipase from Rhizomucor miehei as an Industrial Biocatalyst in Chemical Process, Journal of Molecular Catalysis B: Enzym., Vol. 64, No. 1-2, 1-22.
Naziraa, N. A. J., Salimon, J., Derawi, D., 2016, Enzymatic Glycerolysis of Methyl Laurate Utilizing, Malaysian Journal of Analytical Sciences, Vol. 20, No. 6, 1365-1372.